Figure. 2: 2-DE gel of the non-enzymatic cell wall supernatant (neCWS) from S. aureus strain HIP5827. The sample load was ca. 120 μg protein. The numbers associated with MS-identified spots are listed in column 1 of Table 1 ("A" spots) or Table 2 ("B" spots). The intense train of spots above spot B11 is Chain A, trypsin soybean inhibitor which was added to the sample as a Mr/pI calibrant.