• Periplasmic Proteome of Yersinia pestis KIM 6+
• Staphylococcus aureus Cell Envelope-Associated Proteins
• Staphylococcus aureus Resistance to Vancomycin

The National Institute of Allergy and Infectious Diseases (NIAID) supported Pathogen Functional Genomics Resource Center (PFGRC) designs, constructs, and distributes glass slide DNA microarrays for pathogens and biodefense related organisms (Select A-C agents). Currently, the PFGRC supports DNA microarrays for the 38 organisms listed here. In continuing its efforts to provide the infectious disease and biodefense communities with the microarray resources most relevant to their research efforts, the PFGRC is soliciting input for selection of its next set of reference/species microarrays. The criteria for organism selection may be found here.

Proteomics

The PFGRC Proteomics Facility couples sophisticated protein separation technologies with electrospray and MALDI mass spectrometry to conduct research studies and develop publicly available data sets focused on microbial agents of infectious disease and biodefense. Studies conducted thus far have included a comparative protein abundance analysis of three isogenic Staphylococcus aureus strains derived from a clinical vancomcyin-intermediate S. aureus (VISA) isolate (in collaboration with Emmanuel Mongodin, the J. Craig Venter Institute and Steve Gill, SUNY, Buffalo), the characterization of the periplasmic subcellular proteome of the Yersinia pestis strain KIM6+ in defined medium in vitro and in response to temperature shifts (26°C and 37°C) (in collaboration with Robert Perry, University of Kentucky), and a comparison of in vitro and in vivo proteome profiles, including membrane associated proteins of the enteropathogenic bacterium Shigella dysenteriae (in collaboration with Saul Tzipori, Tufts Cummings School of Veterinary Medicine).

In addition to proteome profiling, the PFGRC Proteomics Group is developing high throughput platforms for in vitro and in vivo protein expression and purification utilizing the Gateway® Destination Vector System. Proteins expressed and purified in this manner will be targeted for specific applications utilizing protein microarrays.

The table below provides links to proteome data sets relevant to each of the studies described above.