- Investigator Login
- Deadlines and Application Procedures
- NIAID PFGRC Site
- NIDCR (Dental Pathogens)
- Available Microarrays
- Description of Annotation Files
- Description of the EASE Archive
- Description of Controls
- Instructions for Downloading Annotations
- Forms and Documentation
- Laboratory Protocols
- Frequently Asked Questions
Three New Bioinformatics Tools Available
The NIAID sponsored Pathogen Functional Genomics Resource Center (PFGRC) at the J. Craig Venter Institute is pleased to announce the release of three new, free open-source software tools: Magnolia, Ginkgo and APEX. Magnolia is a microarray data management and export system for researchers who use PFGRC microarrays. The software greatly simplifies the tasks of organizing experimental data and submitting it to a public data repository. Ginkgo is a Comparative Genomic Hybridization (CGH) and expression microarray data analysis package. Several normalization, data filtering and imputation, and replicate microarray functions are implemented in an intuitive graphical framework. The APEX tool is an implementation of the Absolute Protein Expression quantitation technique. It can compute protein abundance values for LC-MS/MS proteomics datasets, quantifying hundreds or thousands of proteins. Links to additional information on each of these new software tools is available from the PFGRC's bioinformatics page.
Microarray Suggestion Criteria
The National Institute of Allergy and Infectious Diseases (NIAID) supported Pathogen Functional Genomics Resource Center (PFGRC) designs, constructs, and distributes glass slide DNA microarrays for pathogens and biodefense related organisms (Select A-C agents). Currently, the PFGRC supports DNA microarrays for the 38 organisms listed here. In continuing its efforts to provide the infectious disease and biodefense communities with the microarray resources most relevant to their research efforts, the PFGRC is soliciting input for selection of its next set of reference/species microarrays. The criteria for organism selection may be found here.
Available Microarrays
Organism |
Design Strains |
Current Versions |
Older Versions |
Actinobacillus actinomycetemcomitans |
HK1651 |
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Aspergillus fumigatus |
isolate Af293 |
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Aspergillus nidulans |
FGSC A4 |
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Bacillus anthracis |
Ames A2012 |
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Burkholderia mallei/pseudomallei |
ATCC 23344 (mallei) GB8 Horse 4 (mallei) 1710b (pseudomallei) K96243 (pseudomallei) |
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Campylobacter jejuni |
RM1221 81-176 269.97 NCTC 11168 plasmid pCJ1170 (strain RM1170) |
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Candida albicans |
SC5314 |
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Chlamydia trachomatis/pneumoniae |
D/UW-3/CX (serovar D) AR39 CWL029 J138 |
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Clostridium botulinum |
Hall strain A |
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Corona Virus Array |
Human Coronavirus strain 299E SARS Coronavirus strain Tor2 Porcine epidemic diarrhea virus Transmissible gastroenteritis virus Bovine coronavirus Murine hepatitis virus Human coronavirus OC43 Avian infectious bronchitis virus |
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Francisella tularensis |
LVS SCHU 4 pFNL10 (plasmid) pOM1 (plasmid) |
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Fusobacterium nucleatum |
ATCC 25586 ATCC 10953 |
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Giardia lamblia |
WB strain, clone C6 |
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Haemophilus influenzae |
Rd KW20 86-028NP R2846 R2866 pF3031 (biotype aegyptius) pF3028 (biotype aegyptius) F3031 genetic island (biotype aegyptius) HK1212 (Gene discovery) |
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Helicobacter pylori |
26695 J99 |
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Listeria monocytogenes |
EGD-e 1/2a F6854 4b F2365 4b H7858 |
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Mycobacterium smegmatis |
MC2 155 |
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Mycobacterium tuberculosis |
H37Rv CDC1551 |
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Neisseria gonorrhoeae/meningitidis |
FA1090 Z2491 (serogroup A) MC58 (serogroup B) |
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Plasmodium falciparum |
3D7 |
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Plasmodium vivax |
Sal-1 |
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Porphyromonas gingivalis |
W83 |
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Pseudomonas aeruginosa |
PA 01 |
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Rickettsia prowazekii |
Madrid E Wilmington |
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Salmonella typhimurium/typhi |
LT2 CT18 Ty2 |
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Staphylococcus aureus |
COL MU50 MW2 N315 MRSA252 MSSA476 pLW043 |
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Streptococcus agalactiae |
NEM316 2603V/R A909 |
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Streptococcus mutans |
UA159 |
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Streptococcus pneumoniae |
TIGR4 R6 G54 Bacteriophage MM1 |
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Streptococcus pyogenes |
SSI-1 MGAS315 MGAS2096 MGAS5005 MGAS6180 MGAS8232 MGAS9429 MGAS10270 MGAS10394 MGAS10750 SF370 plasmid pDN571 (71-724) |
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Streptococcus sanguinis |
SK36 |
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Test Microarray |
Streptococcus pneumoniae TIGR4 Staphylococcus aureus COL Salmonella typhimurium LT2 |
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Treponema denticola |
ATCC 35405 |
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Trypanosoma brucei |
TREU927 |
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Trypanosoma cruzi |
CL Brener TC3 |
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Vibrio cholerae |
el tor N16961 |
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Vibrio parahaemolyticus |
AQ3810 RIMD 2210633 plasmid pO3K6 (strain KXV237) |
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Vibrio vulnificus |
YJ016 CMCP6 |
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Yersinia pestis |
CO-92 KIM |
