October 7, 2010
The PFGRC announces the addition of an Enterotoxigenic E. coli (ETEC) Expression Clone Set to the PFGRC Gateway® Entry Clone Resource
The National Institute of Allergy and Infectious Diseases (NIAID) sponsored Pathogen Functional Genomics Resource Center (PFGRC) at JCVI is pleased to announce the addition of an Enterotoxigenic E. coli (ETEC) Expression Clone Set to the PFGRC Gateway® Entry Clone Resource. This clone set was produced as the result of a collaboration between Dr. Philip Hardwidge at University of Kansas Medical Center and the PFGRC at JCVI. The clone set consists of 917 full-length ORFs and ORF fragments and was derived from three strains B7A, E24337A, and H10407. The clones were constructed in the pMCSG7 expression vector using a ligation independent cloning method. Each ORF has an introduced universal ATG start codon and a native/introduced stop codon and codes for an N-terminal 6X HisTag fusion. The resource is currently available to the research community free of charge through BEI Resources.