July 16, 2009
M. tuberculosis knock-out clones now available through the PFGRC Gateway® Entry Clone Resource
The National Institute of Allergy and Infectious Diseases (NIAID)sponsored Pathogen Functional Genomics Resource Center (PFGRC) at JCVI,in a white paper collaboration with the Johns Hopkins School of Medicine, Department of Medicine, Division of Infectious Diseases, is pleased to announce that it is making available 615 knock-out clones for Mycobacterium tuberculosis strain CDC1551, available through the PFGRC Gateway® Entry Clone Resource. These knock-out clones target non-operonic non-essential ORFs that are not represented in the Himar1 transposon-mediated knockout library (Sassetti et al.).
Invitrogen's three-fragment MultiSite Gateway® Pro method was used to assemble the selectable marker between flanking chromosomal regions. The hygromycin selectable marker has been sequence verified and the final constructs have been confirmed by restriction digest mapping.
The PFGRC is making this resource available to the research community free of charge as individual clones. Please follow the link to the About Gateway® Custom Knockout Clones for detailed ordering instructions for individual Clones.