Three New Bioinformatics Tools Available
The NIAID sponsored Pathogen Functional Genomics Resource Center (PFGRC) at the J. Craig Venter Institute is pleased to announce the release of three new, free open-source software tools: Magnolia, Ginkgo and APEX. Magnolia is a microarray data management and export system for researchers who use PFGRC microarrays. The software greatly simplifies the tasks of organizing experimental data and submitting it to a public data repository. Ginkgo is a Comparative Genomic Hybridization (CGH) and expression microarray data analysis package. Several normalization, data filtering and imputation, and replicate microarray functions are implemented in an intuitive graphical framework. The APEX tool is an implementation of the Absolute Protein Expression quantitation technique. It can compute protein abundance values for LC-MS/MS proteomics datasets, quantifying hundreds or thousands of proteins. Links to additional information on each of these new software tools is available from the PFGRC's bioinformatics page.
Microarray Suggestion Criteria
The National Institute of Allergy and Infectious Diseases (NIAID) supported Pathogen Functional Genomics Resource Center (PFGRC) designs, constructs, and distributes glass slide DNA microarrays for pathogens and biodefense related organisms (Select A-C agents). Currently, the PFGRC supports DNA microarrays for the 38 organisms listed here. In continuing its efforts to provide the infectious disease and biodefense communities with the microarray resources most relevant to their research efforts, the PFGRC is soliciting input for selection of its next set of reference/species microarrays. The criteria for organism selection may be found here.
January 20, 2006
The PFGRC and Harvard Institute of Proteomics announce the availability of three new additions to the PFGRC Invitrogen Gateway® Entry Clone Resource.
Details with respect to the construction and ordering the S. aureus, V. cholerae, and F. tularensis Gateway® Entry Clones can be found below.
The PFGRC Announces the Availability of a Staphylococcus aureus Gateway® Entry Clone Set.
The National Institute of Allergy and Infectious Diseases (NIAID) sponsored Pathogen Functional Genomics Resource Center (PFGRC) at TIGR is pleased to announce the addition of a Staphylococcus aureus strain COL Invitrogen Gateway® Entry Clone Set to the PFGRC Gateway® Entry Clone Resource. The S. aureus ORFeome library was prepared and sequence validated in its entirety by the PFGRC. The resulting entry clone collection consists of 2,400 validated open reading frames constructed in the pDONR221 Invitrogen Gateway® Entry Clone Vector with modified att sites. The PFGRC is making this resource available to the research community free of charge as a complete clone set or individual entry clones. Please follow the link to the PFGRC Invitrogen Gateway® Entry Clone Resource for detailed ordering instructions for the complete clone set or individual clones.
The PFGRC and Harvard Institute of Proteomics Announce the Availability of a Vibrio cholerae Gateway® Entry Clone Set.
The National Institute of Allergy and Infectious Diseases (NIAID) sponsored Pathogen Functional Genomics Resource Center (PFGRC) at TIGR is pleased to announce the addition of a Vibrio cholerae strain N16961, biotype ElTor Invitrogen Gateway® Entry Clone Set to the PFGRC Gateway® Entry Clone Resource. The V. cholerae ORFeome library was prepared and full-length sequence verified by the Harvard Institute of Proteomics with support from NIAID. The resulting Entry clone collection consists of 2983 validated open reading frames constructed in the pDONR221 Invitrogen Gateway® Entry Clone Vector with modified att sites. The PFGRC is making this resource available to the research community free of charge as a complete clone set or individual entry clones. Please follow the link to the PFGRC Invitrogen Gateway® Entry Clone Resource for detailed ordering instructions for the complete clone set or individual clones.
The PFGRC Announces the Availability of a F. tularensis Gateway® Entry Clone Set.
The National Institute of Allergy and Infectious Diseases (NIAID) sponsored Pathogen Functional Genomics Resource Center (PFGRC) at TIGR is pleased to announce the addition of a Francisella tularensis strain Schu S4 Invitrogen Gateway® Entry Clone Set to the PFGRC Gateway® Entry Clone Resource. The F. tularensis ORFeome library was prepared and sequence validated in its entirety by the PFGRC. The resulting entry clone collection consists of 1475 validated open reading frames constructed in the pDONR221 Invitrogen Gateway® Entry Clone Vector with modified att sites. The PFGRC is making this resource available to the research community free of charge as a complete clone set or individual entry clones. Please follow the link to the PFGRC Invitrogen Gateway® Entry Clone Resource for detailed ordering instructions for the complete clone set or individual clones.