The National Institute of Allergy and Infectious Diseases (NIAID) sponsored Pathogen Functional Genomics Resource Center (PFGRC) at The Institute for Genomic Research (TIGR) has constructed and is making available a clone set called the SARS-CoV Gateway® Clone Set. This clone set consists of twenty seven (27) clones containing ORFs based on the Genbank accession number NC_004718.3. Each clone in the set is clearly identified by its accession. The clones are constructed in the vector pDONR 221, (Invitrogen, Inc). These "entry clones" are constructed such that in-frame expression constructs can be readily produced through the use of Invitrogen's Gateway® Cloning Technology. This is accomplished by performing a recombination reaction using LR Clonase (Invitrogen, cat#s 11791019, 11791043).

It is recommended that investigators unfamiliar with Invitrogen's Gateway® Cloning Technology consult the educational website for further information.

Each entry clone has been sequenced using a combination of end sequencing and primer walking in an attempt to determine each base at an average of 2-fold coverage. Sequence mutations (if any) introduced during the PCR amplification process, are generally identified by the PFGRC's sequence validation process. Specific data pertaining to each clone can be found on the PFGRC web site.