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Invitrogen Gateway® Entry Clone Resource

Mycobacterium tuberculosis H37Rv / CDC 1551

Clone Library Features

Creation Date:

December 2006

Sequence Validated Clones:

2,968

Entry Clone Vector:

pDONR221

Start Codons:

ATG standardized

Stop Codons:

Absent

att Sites:

Modified

attB1:

5' CAACT-TTG-TAC-AAA-AAA-GTT-GCC-CAT-ATG

attB2:

5' CAAC-TTT-GTA-CAA-GAA-AGT-TGC-AAG-CTT


Distribution Information

Glycerol Stock Cell Type:

DH10B-T1 E. coli in 25% (by volume) glycerol

Distribution Format:

(31) 96-well, V-bottom microtiter plates


Validation Requirements

Minimum 1x coverage

Mutation rate < 0.2% in CDS sequence

No missense or frameshift mutations