Invitrogen Gateway® Entry Clone Resource
SARS-Coronavirus
Clone Library Features |
|
GenBank Accession#: |
NC_004718 |
Creation Date: |
May 2003 |
Sequence Validated Clones: |
27 |
Entry Clone Vector: |
pDONR221 |
Start Codons: |
ATG (standardized) |
Stop Codons: |
Absent |
att Sites: |
PFGRC Standard |
attB1: |
5' CAAGT-TTG-TAC-AAA-AAA-GCA-GGC-TTC |
attB2: |
5' CCAC-TTT-GTA-CAA-GAA-AGC-TGG-GTC |
Distribution Information |
|
Glycerol Stock Cell Type: |
DH10B-T1 E. coli in 25% (by volume) glycerol |
Distribution Format: |
(27) Individually sealed vials |
Validation Requirements |
Minimum 1x coverage |
Mutation rate < 0.2% in CDS sequence |
No missense or frameshift mutations |
Additional Notes |
Three (3) cloned genes (ORFs NP_828851, NP_828869 and NP_828869b) are contained in an Invitrogen E. coli strain that must be grown at 30°C and not 37°C. See SARS guidelines for details. |